Composite

Part:BBa_K3861024

Designed by: Kristin Funke   Group: iGEM21_Humboldt_Berlin   (2021-10-01)


PlldR-sicP-sptP-Pep8


Composite part consisting of BBa_K3861000 and BBa_K3861010. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI-1 T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal PstI site found at 519
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None